Which diagnostic test is most appropriate to confirm Enterobius vermicularis infection?

• A. Stool culture
• B. Scotch tape test
• C. Blood smear
• D. Urine analysis

Answer: (B)

The test relies on detecting pinworm eggs that are laid around the anus during the night. Because the eggs are deposited perianally rather than shed in stool, an adhesive tape method collecting from the skin near the anus is the most sensitive way to confirm infection. The Scotch tape test captures these eggs when pressed onto the perianal area and then examined under a microscope.

Stool culture isn’t reliable for pinworm because eggs are not consistently present in stool. Blood smear and urine analysis don’t help diagnose pinworm infections, as they assess other systems and organisms.

Practical tip: collect the tape sample in the morning before bathing or defecation, stick it to a glass slide, and examine for pinworm eggs. In some cases, multiple consecutive samples increase the chance of detection.